"I stand with President Trump, I stand with our soldiers, and I will always stand for our flag and our national anthem", he added.
Trump angered NFL stars, team owners and league officials last month after describing players who had protested against racial injustice by refusing to stand for the anthem as "sons of bitches".
Trump said in a tweet that he had asked Pence to leave the stadium if any players kneeled during the national anthem, claiming such actions were disrespectful.
In Detroit, the Lions stood arm in arm, including two players who had knelt the past two weeks.
Republican vice presidential candidate, Indiana Governor Mike Pence speaks to supporters at a rally in Chesterfield, Missouri.
Mr. Trump most recently suggested team owners are reluctant to address players' protests because they're "afraid" of their players.
Earlier Sunday, Pence, the former governor of IN, tweeted a picture of he and his wife Karen decked out IN Colts sportswear.
It was announced Friday that Pence would attend the game with the second lady.
The controversial practice of taking a knee was started by former-San Francisco quarterback Colin Kaepernick, who has yet to be signed to a new team after his contract with the 49ers expired previous year. We spoke at length about numerous issues that face our community, including systemic oppression against people of color, police brutality and the criminal justice system.
The protest began in August 2016, when former 49ers quarterback Colin Kaepernick sat during the national anthem in preseason games.
President Donald Trump tweeted afterward that he asked Pence to leave the stadium.
The vice president went on to issue a full statement opposing the protest.
However, a few hours later he updated his Twitter saying that he left the game following the display during the national anthem.