She had been asked by the defense to identify Holly's driving licence and describe what else was in her wallet on the day she was murdered when she fell off her chair, sobbing: 'I feel like I can't breathe'.
Holly's mother, Karen, said she felt sick and suddenly collapsed on the witness stand.
Bobo's remains would be found three years later, in September, 2014.
Prosecutor Paul Hagerman claimed that Zachary Adams was on methamphetamine and morphine when he allegedly kidnapped, raped, and killed Bobo, WREG-TV reported.
Two other men were also charged in her killing but one was granted immunity in exchange for his testimony against Adams. Her brother assumed at the time that she was with her boyfriend. The Holly Bobo case trial is expected to begin on Monday, Sept. 11.
About 50 to 60 blood drops were found on the floor of the carport.
Bobo lived with her parents in a rural community about 150 miles west of Nashville.
Jurors listened to a 911 call from April 2011 and looked at several pieces of evidence, including the purse and lunch bag Holly carried before she disappeared. The trial, which was originally scheduled to begin in July, was postponed until September, to give the defense more time to examine the weapon. The judge refused it. She said she last saw her daughter studying in the kitchen at the family's Decatur County home.
Bobo said he spoke with his mother, Karen, on the phone. She called Clint straight away, she said, and he said he thought the man his sister was with was her boyfriend, Drew Scott.
Zach Adams is charged with kidnapping, raping and killing Decatur County nursing student Holly Bobo.
Karen Bobo said on the morning Holly disappeared, someone told her they got a call from a neighbor saying they heard screams coming from their home.
His brother Dylan Adams is also accused in the case along with Jason Autry.
Holly's father Dana (L) and brother Clint (R) also testified on Monday. Afterwards, he rolled her body up in a carpet and put her in his truck after beating her, he said. He reportedly told Autry that he was going to "gut" her body so it wouldn't float when tossed into the Tennessee River. While she was wrapped in a sheet in his vehicle, he realized she was not yet dead and shot her in the head, prosecutors said. No bones below her neck have ever been found, sources say. So far, six men have been arrested in connection with her death, but only three of them have been prosecuted. John Adams however retracted his initial confession shortly after making it, claiming it was coerced.
Five people have been granted either federal or state immunity to testify against Adams.
Some of those questions included feelings on the charges Adams faces, which are felony first-degree murder, especially aggravated kidnapping and aggravated rape, the types of witnesses who could be called to testify, and how the knowledge of promises made to witnesses who have knowledge about the crime. The expert told the jury that indicated to him there was a struggle.
The trial in Savanna, Tennessee, continues.